Int J Biol Sci 2012; 8(10):1335-1344. doi:10.7150/ijbs.4654

Research Paper

Stem Cell Transplantation Increases Antioxidant Effects in Diabetic Mice

Ming Li 1, Luca Vanella 2, Yuming Zhang 3, Ming Shi 1, Takashi Takaki4, Joseph I Shapiro2, Susumu Ikehara1✉

1. Department of Stem Cell Disorders, Kansai Medical University, Moriguchi City, Osaka, Japan.
2. School of Medicine, University of Marshall, 1600 Medical Center Drive, Huntington, WV 25701, USA.
3. Department of Pediatrics, Nanfang Hospital, Guangzhou, China.
4. JEOL Ltd., Tokyo, Japan.

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Li M, Vanella L, Zhang Y, Shi M, Takaki T, Shapiro JI, Ikehara S. Stem Cell Transplantation Increases Antioxidant Effects in Diabetic Mice. Int J Biol Sci 2012; 8(10):1335-1344. doi:10.7150/ijbs.4654. Available from

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Intra bone marrow-bone marrow transplantation (IBM- BMT) + thymus transplantation (TT) has been shown to reduce the incidence of graft versus host disease (GVHD) and restore donor-derived T cell function. In addition, an increase in insulin sensitivity occurred in db/db mice after IBM-BMT+TT treatment. Heme oxygenase (HO)-1 is a stress inducible enzyme which exert antioxidant, antiapoptotic, and immune-modulating properties. We examined whether IBM-BMT+TT could modulate the expression of HO-1 in the kidneys of db/db mice. Six-week-old db/db mice with blood glucose levels higher than 250 mg/dl were treated with IBM-BMT+TT. Six weeks later, the db/db mice showed decreased body weight, blood glucose levels and insulin, and increased plasma adiponectin levels. The upregulation of HO-1 was associated with significantly (p<0.05) increased levels of peNOS and pAKT, but decreased levels of iNOS in the kidneys of db/db mice. Plasma creatinine levels also decreased (p<0.05), and the expression of type IV collagen was improved. Thus IBM-BMT+TT unregulated the expression of HO-1, peNOS and pAKT, while decreasing iNOS levels in the kidney of db/db mice. This was associated with an improvement in renal function.

Keywords: IBM-BMT+TT, antioxidant, HO-1, diabetic nephropathy.