Int J Biol Sci 2020; 16(11):1833-1845. doi:10.7150/ijbs.41940

Research Paper

Ozone Therapy Attenuates NF-κB-Mediated Local Inflammatory Response and Activation of Th17 Cells in Treatment for Psoriasis

Jinrong Zeng1*, Li Lei1*, Qinghai Zeng1, Yuying Yao2, Yuqing Wu2, Qinxuan Li2, Lihua Gao1, Hongjiao Du1, Yajie Xie1, Jinhua Huang1, Wenbin Tan3,4, Jianyun Lu1✉

1. Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha, Hunan, China;
2. XiangYa School of Medicine, Central South University, Changsha, Hunan, China;
3. Department of Cell Biology and Anatomy, School of Medicine, and
4. Department of Biomedical Engineering, College of Engineering and Computing, University of South Carolina, Columbia, South Carolina
*These authors contributed equally to this work.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Citation:
Zeng J, Lei L, Zeng Q, Yao Y, Wu Y, Li Q, Gao L, Du H, Xie Y, Huang J, Tan W, Lu J. Ozone Therapy Attenuates NF-κB-Mediated Local Inflammatory Response and Activation of Th17 Cells in Treatment for Psoriasis. Int J Biol Sci 2020; 16(11):1833-1845. doi:10.7150/ijbs.41940. Available from http://www.ijbs.com/v16p1833.htm

File import instruction

Abstract

Ozone therapy has been widely used to treat many skin diseases, including infections, allergic dermatosis, and skin ulcers. However, its efficacy as a treatment for psoriasis is unclear. In this study, we explored the clinical efficacy and the underlying molecular mechanisms of ozone therapy on psoriasis. We found that topical ozone treatment significantly decreased patients' psoriasis area and severity index (PASI) scores and the expression of psoriasis-associated cytokines in their peripheral blood CD4+ T cells. In the IMQ-induced psoriasis mouse model, topical ozone treatment significantly inhibited the formation of IMQ-induced psoriasis-like lesions and the expression of psoriasis-associated inflammatory factors. High-throughput sequencing confirmed that IMQ-induced activation of toll-like receptor 2 (TLR2)/ nuclear factor-κB (NF-κB) signaling pathway was significantly suppressed in psoriasis-like lesions after topical ozone treatment. Furthermore, the activation of spleen T helper (Th) 17 cells was blocked in the mouse model; this was associated with the downregulation of cytokines and NF-κB pathways upon topical ozone treatment. Ozone therapy can attenuate local inflammatory reactions and the activation of Th17 cells in psoriasis by inhibiting the NF-κB pathway. Our results show that ozone therapy is effective in treating psoriasis. We recommend further evaluations for its clinical applications.

Keywords: ozone therapy, NF-κB, TLR2, Th17, psoriasis