Int J Biol Sci 2011; 7(1):53-60. doi:10.7150/ijbs.7.53
Intratumoral Expression of IL-17 and Its Prognostic Role in Gastric Adenocarcinoma Patients
1. The State Key Laboratory of Oncology in Southern China and Department of Experimental Research, Cancer Center, Sun Yat-sen University, Guangzhou 510060, P.R. China.
2. State Key Laboratory of Oncology in South China, 651 Dongfeng Road East, Guangzhou 510060, China; Department of Gastric and Pancreatic Surgery, Sun Yat-sen University Cancer Center, 651 Dongfeng Road East, Guangzhou 510060, China.
3. Immunotherapy Asia Center, Kuramae-kaikan 4F, 3-1-1 Kuramae, Taito-ku, Tokyo 111-0051, Japan.
4. Department of Surgery, University of Michigan Medical Center, 1520A MSRB-1, Ann Arbor, Michigan 48109-0666, USA.
Chen Jg, Xia Jc, Liang Xt, Pan K, Wang W, Lv L, Zhao Jj, Wang Qj, Li Yq, Chen Sp, He J, Huang Lx, Ke Ml, Chen Yb, Ma Hq, Zeng Zw, Zhou Zw, Chang AE, Li Q. Intratumoral Expression of IL-17 and Its Prognostic Role in Gastric Adenocarcinoma Patients. Int J Biol Sci 2011; 7(1):53-60. doi:10.7150/ijbs.7.53. Available from http://www.ijbs.com/v07p0053.htm
In this study, we characterized the intratumoral expression of IL-17 and CD8+ TILs in gastric adenocarcinoma patients after resection and determined the correlation between the survival probability of gastric adenocarcinoma patients and the expression of IL-17 in tumor. Expression of IL-17 and CD8 was assessed by immunohistochemistry, and the prognostic effects of intratumoral IL-17 expression and CD8+ TILs were evaluated by Cox regression and Kaplan-Meier analysis. Immunohistochemical detection revealed the presence of IL-17 and CD8+ cells in gastric adenocarcinoma tissue samples (90.6%, 174 out of 192 patients and 96.9%, 186 out of 192 patients, respectively). We have also found that intratumoral IL-17 expression was significantly correlated with age (p=0.004) and that the number of CD8+ TILs was significantly correlated with UICC staging (p=0.012) and the depth of tumor invasion (p=0.022). The five-year overall survival probability among patients intratumorally expressing higher levels of IL-17 was significantly better than those expressing lower levels of IL-17 (p=0.036). Multivariate Cox proportional hazard analyses revealed that intratumoral IL-17 expression (HR: 0.521; 95% CI: 0.329-0.823; p=0.005) was an independent factor affecting the five-year overall survival probability. We conclude that low levels of intratumoral IL-17 expression may indicate poor prognosis in gastric adenocarcinoma patients.
Keywords: IL-17, intratumoral expression, gastric carcinoma, prognostic indicator