Isolation and Identification of Insect Intestinal Mucin Haiim86 - The New Target for <i>Helicoverpa Armigera</i> Biocontrol

Zhang, Xia; Guo, Wei

doi:10.7150/ijbs.7.286



Theranostics

International Journal of Medical Sciences

Nanotheranostics

Journal of Cancer

Journal of Genomics

Global reach, higher impact

Full Text | PDF

Int J Biol Sci 2011; 7(3):286-296. doi:10.7150/ijbs.7.286 This issue Cite

Research Paper

Isolation and Identification of Insect Intestinal Mucin Haiim86 - The New Target for Helicoverpa Armigera Biocontrol

Xia Zhang^{1, 2}, Wei Guo^{1 ✉}

1. Biological Control Centre of Plant Pathogens and Plant Pests of Hebei Province, College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China.
2. Biological Institute, Hebei Academy of Science, Shijiazhuang 050051, China

Citation:

Zhang X, Guo W. Isolation and Identification of Insect Intestinal Mucin Haiim86 - The New Target for Helicoverpa Armigera Biocontrol. Int J Biol Sci 2011; 7(3):286-296. doi:10.7150/ijbs.7.286. https://www.ijbs.com/v07p0286.htm

Other styles

Abstract

There are many more glycoproteins in Helicoverpa armigera peritrophic membrane than midgut separated by SDS-PAGE analysis after Periodic acid-Schiff (PAS) and coomassie staining. The peritrophic membrane (PM) of H. armigera larvae contains about forty associated proteins. A cDNA library was constructed from H. armigera midgut mRNA to study the new target for pest biocontrol. An antiserum against Spodoptera exigua integral/total PM proteins cross reacted with several H. armigera PM proteins and was used to isolate a complete cDNA encoding an insect intestinal mucin (HaIIM86). The deduced protein sequence of the cDNA contained one potentially glycosylated, mucin-like domain, five cysteine-rich chitin-binding domains (CBDs) and two D-G rich regions. Mucin domain was lined between the first and second CBDs; the two additional D-G rich regions were proposed to internal reside at the amino terminus of the protein flanked by three cysteine-rich CBDs. HaIIM86 contains two D-G-rich tandem repeat domains flanked by cysteine-rich sequences in peritrophic membrane proteins which is not present in all the currently known PM proteins. Howerer the functions of D-G rich domains require further investigation. HaIIM86 was shown as 200kDa protein by SDS-PAGE analysis and appeared to be associated with the PM. HaIIM86 has chitin-binding activity and can be degraded into 90 and 70 kDa by HaGV Enhancin in vivo. The finding has shown that HaIIM86 is the target substrate for enhancin and the potential target for pest control.

Keywords: Peritrophic matrix, Helicoverpa armigera, cDNA expression library, insect intestinal mucin, biocontrol

Citation styles

APA

Zhang, X., Guo, W. (2011). Isolation and Identification of Insect Intestinal Mucin Haiim86 - The New Target for Helicoverpa Armigera Biocontrol. International Journal of Biological Sciences, 7(3), 286-296. https://doi.org/10.7150/ijbs.7.286.

ACS

Zhang, X.; Guo, W. Isolation and Identification of Insect Intestinal Mucin Haiim86 - The New Target for Helicoverpa Armigera Biocontrol. Int. J. Biol. Sci. 2011, 7 (3), 286-296. DOI: 10.7150/ijbs.7.286.

NLM

CSE

Zhang X, Guo W. 2011. Isolation and Identification of Insect Intestinal Mucin Haiim86 - The New Target for Helicoverpa Armigera Biocontrol. Int J Biol Sci. 7(3):286-296.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.