Int J Biol Sci 2011; 7(5):496-504. doi:10.7150/ijbs.7.496

Research Paper

Overexpression of Grb2-associated Binder 2 in Human Lung Cancer

Xiu-Li Xu1, Xia Wang1, Zhao-Li Chen2 ✉, Min Jin2, Wu Yang2, Guo-Fan Zhao 1, Jun-Wen Li2 ✉

1. Department of Pathology, Tianjin Medical University, Heping District, Tianjin 300070, China;
2. Institute of Environment and Health; Key Laboratory of Risk Assessment and Control for Environment & Food Safety, No.1, Dali Road, Tianjin 300050, China.

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Citation:
Xu XL, Wang X, Chen ZL, Jin M, Yang W, Zhao GF, Li JW. Overexpression of Grb2-associated Binder 2 in Human Lung Cancer. Int J Biol Sci 2011; 7(5):496-504. doi:10.7150/ijbs.7.496. Available from http://www.ijbs.com/v07p0496.htm

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Abstract

Objective: Grb2-associated binder 2 (Gab2), a member of the family of Gab scaffolding adaptors, transmits and amplifies the signals from receptor tyrosine kinases. A recent study demonstrated that Gab2 was over-expressed in breast cancers and metastatic melanomas, and Gab2 was an oncogenic protein. However, the roles of Gab2 in lung cancers are largely unknown.

Method: In this study, to investigate whether Gab2 expression could be a characteristic of lung cancers, we analyzed the expression of Gab2 in 88 lung frozen tissue samples and 122 paraffin-embedded tissue specimens, using quantitative real-time-PCR, immunohistochemistry and western blot.

Results: We found that the positive expression rate of Gab2 in the tumor tissues, as detected by immunohistochemistry, 62.5% in squamous cell cancers, 51.35% in adenocarcinomas, and 75% in other types of lung cancers, was significantly higher than that (12%) in normal lung tissues. The mRNA expression detected by quantitative real-time-PCR and protein expression detected by western blotting in different groups were consistent with the immunohistochemical results.

Conclusion: Our data indicate that Gab2 is over-expressed in malignant lung tissues compared with that in normal lung tissues, and suggest that Gab2 expression may play a role in lung cancer development.

Keywords: Gab2, lung cancer, quantitative real-time-PCR, immunohistochemistry, western blot