Int J Biol Sci 2019; 15(5):1104-1112. doi:10.7150/ijbs.31794
Taurine Transporter Regulates Adipogenic Differentiation of Human Adipose-Derived Stem Cells through Affecting Wnt/β-catenin Signaling Pathway
1. Suzhou Institute of Systems Medicine, Center of Systems Medicine, Chinese Academy of Medical Sciences, Suzhou215123, Jiangsu, China
2. Department of Burn and Plastic Surgery, North District of Suzhou Municipal Hospital, Suzhou 215008, Jiangsu, China
3. Department of Pharmacology, College of Pharmacy, Second Military Medical University, Shanghai 200433, China
4. Department of Emergency, The First Affiliated Hospital of Soochow University, Suzhou, 215006, Jiangsu Province, China.
*These authors contributed equality.
Hou X, Wang Z, Ding F, He Y, Wang P, Liu X, Xu F, Wang J, Yang Y. Taurine Transporter Regulates Adipogenic Differentiation of Human Adipose-Derived Stem Cells through Affecting Wnt/β-catenin Signaling Pathway. Int J Biol Sci 2019; 15(5):1104-1112. doi:10.7150/ijbs.31794. Available from http://www.ijbs.com/v15p1104.htm
Increased adipocytes are associated with obesity and many human disorders including cancers. To further understand the molecular mechanisms of adipogenesis, transcriptome sequencing was performed to find genes involved in the adipogenic differentiation of human adipose-derived stem cells (hASCs). The mRNA of taurine transporter (TauT, also known as SLC6A6) was found significantly upregulated in hASCs undergoing differentiation. TauT expression was also markedly increased in fat tissues from obese mice induced by high fat diet or genetic mutations (ob/ob and db/db mice). In vitro, downregulation of TauT attenuated effectively the adipogenic differentiation of hASCs, and TauT overexpression promoted the formation of adipocytes. Among the molecules transported by TauT, hypotaurine and β-alanine promoted adipocyte formation, whereas taurine inhibited the process. Moreover, the inhibitory effect of TauT knockdown on hASCs differentiation was largely reversed by hypotaurine and β-alanine through promoting the downregulation of β-catenin. These results indicated that TauT regulate adipocyte formation through transported amino acids and may serve as a target for therapeutic intervention of obesity.
Keywords: human adipose-derived stem cells, taurine transporter, adipogenic differentiation