Int J Biol Sci 2019; 15(11):2340-2349. doi:10.7150/ijbs.34010 This issue
1. State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, 410081, China
2. College of Life Sciences, Hunan Normal University, Changsha, Hunan, 410081, China
3. School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China
4. College of Biology and Environmental Engineering, Changsha University, Changsha, 410081, China
*These authors contributed equally to this work.
Induced pluripotent stem (iPS) cells provide a powerful platform for the study of development, regeneration, and disease. Although many stable iPS cell lines have been established for mammals, few attempts have been made to induce iPS cells in nonmammalian species. Because of technical advantages over other vertebrates on stem cells, induced pluripotent stem cells from fish could be of value for research. In this paper, stable iPS-like cell lines were generated from adult zebra fish fibroblasts by combining the doxycycline inducible lentiviral delivery system and chemical treatment. RT-PCR analysis, alkaline phosphatase staining, and immunofluorescence indicated that adult zebra fish fibroblasts were successfully reprogrammed into iPS-like cells (ziPSCs). The ziPSCs exhibited stable growth and manifested many features of fish embryonic stem cells with pluripotency in vitro and in vivo. Because of easy maintenance, the developed technology in this study for generating zebra fish iPS-like cells could be extended to investigating other genera of fish.
Keywords: induced pluripotent stem cell, doxycycline, zebra fish, fibroblast, lentiviral delivery system