Drp1-mediated mitochondrial fission contributes to baicalein-induced apoptosis and autophagy in lung cancer via activation of AMPK signaling pathway

Deng, Xiaohong; Liu, Jingjing; Liu, Lantao; Sun, Xianjun; Huang, Jianhua; Dong, Jingcheng

doi:10.7150/ijbs.41768

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Theranostics

International Journal of Medical Sciences

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Journal of Cancer

Journal of Genomics

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Int J Biol Sci 2020; 16(8):1403-1416. doi:10.7150/ijbs.41768 This issue Cite

Research Paper

Drp1-mediated mitochondrial fission contributes to baicalein-induced apoptosis and autophagy in lung cancer via activation of AMPK signaling pathway

Xiaohong Deng¹, Jingjing Liu¹, Lantao Liu², Xianjun Sun¹, Jianhua Huang¹, Jingcheng Dong^1✉

1. Department of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China
2. Department of Interventional Radiology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200050, China

Citation:

Deng X, Liu J, Liu L, Sun X, Huang J, Dong J. Drp1-mediated mitochondrial fission contributes to baicalein-induced apoptosis and autophagy in lung cancer via activation of AMPK signaling pathway. Int J Biol Sci 2020; 16(8):1403-1416. doi:10.7150/ijbs.41768. https://www.ijbs.com/v16p1403.htm

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Abstract

Baicalein (BA), a natural compound extracted from Scutellaria baicalensis Georgi, has been reported to exert antitumor effect in various cancers. However, the underlying mechanisms have not been well demonstrated. In the present study, we focused on the relationship between mitochondrial fission and BA-induced apoptosis and autophagy. We showed that BA inhibited cell viability and induced mitochondrial apoptosis in A549 and H1299 lung cancer cells. BA induced the loss of mitochondrial membrane potential (MMP) and the release of cytochrome c and apoptosis inducing factor (Aif) from mitochondria to cytoplasm. Meanwhile, BA induced autophagy and activated autophagic flux. Furthermore, we found that BA induced mitochondrial fission and mitochondrial impairment. Blocking mitochondrial fission by mdivi-1 attenuated BA-induced apoptosis and autophagy. Moreover, BA activated AMP-activated protein kinase (AMPK) pathway. Knockdown of AMPK with lentivirus encoded AMPKα also attenuated BA-induced mitochondrial fission, apoptosis and autophagy. Our in vivo data confirmed that BA inhibited tumor growth and induced apoptosis and autophagy in a Lewis lung carcinoma (LLC) xenograft model via activation of AMPK/mitochondrial fission pathway. Our study highlights the critical role of AMPK/mitochondrial fission pathway in the regulation of BA-induced apoptosis and autophagy. These results revealed the molecular mechanism of the anti-lung cancer property of BA and provided novel perspectives for the application of BA in the treatment of lung cancer.

Keywords: baicalein, mitochondrial fission, apoptosis, autophagy, AMPK

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APA

Deng, X., Liu, J., Liu, L., Sun, X., Huang, J., Dong, J. (2020). Drp1-mediated mitochondrial fission contributes to baicalein-induced apoptosis and autophagy in lung cancer via activation of AMPK signaling pathway. International Journal of Biological Sciences, 16(8), 1403-1416. https://doi.org/10.7150/ijbs.41768.

ACS

Deng, X.; Liu, J.; Liu, L.; Sun, X.; Huang, J.; Dong, J. Drp1-mediated mitochondrial fission contributes to baicalein-induced apoptosis and autophagy in lung cancer via activation of AMPK signaling pathway. Int. J. Biol. Sci. 2020, 16 (8), 1403-1416. DOI: 10.7150/ijbs.41768.

NLM

CSE

Deng X, Liu J, Liu L, Sun X, Huang J, Dong J. 2020. Drp1-mediated mitochondrial fission contributes to baicalein-induced apoptosis and autophagy in lung cancer via activation of AMPK signaling pathway. Int J Biol Sci. 16(8):1403-1416.

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