Int J Biol Sci 2020; 16(11):1821-1832. doi:10.7150/ijbs.37552

Research Paper

A novel vehicle-like drug delivery 3D printing scaffold and its applications for a rat femoral bone repairing in vitro and in vivo

Hui Wang1*, Zhengwei Deng2,5*, Jing Chen2,5*, Xin Qi2, Libing Pang3, Bocai Lin1, Yan Teik Yuin Adib1,4, Na Miao6, Deping Wang3, Yadong Zhang2,5✉, Jiusheng Li1 ✉, Xiangqiong Zeng1✉

1. Laboratory for Advance Lubricating Materials, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
2. Department of Orthopedics, Fengxian District Central Hospital Affiliated of Shanghai University of Medicine&Health Sciences, 279 zhouzhu road, Shanghai 220120, People's Republic of China.
3. School of Materials Science and Engineering, Tongji University, Shanghai 201804, China.
4. School of Life Science & Chemical Technology, Ngee Ann Polytechnic, Singapore 599489
5. Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
6. Department of Pediatrics, Maternal and Child Health Hospital of Zaozhuang City, Shandong, China.
*The first authors contributed equally to this work

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Citation:
Wang H, Deng Z, Chen J, Qi X, Pang L, Lin B, Adib YTY, Miao N, Wang D, Zhang Y, Li J, Zeng X. A novel vehicle-like drug delivery 3D printing scaffold and its applications for a rat femoral bone repairing in vitro and in vivo. Int J Biol Sci 2020; 16(11):1821-1832. doi:10.7150/ijbs.37552. Available from http://www.ijbs.com/v16p1821.htm

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Abstract

The high surface area ratio and special structure of mesoporous bioactive glass (MBG) endow it with excellent physical adsorption of various drugs without destroying the chemical activity. Silicate 1393 bioactive glass (1393) is famous for its fantastic biodegradability and osteogenesis. Herein, we have built a novel vehicle-like drug delivery 3D printing scaffold with multiplexed drug delivery capacity by coating MBG on the surface of 1393 (1393@MBG). Furthermore, we have applied DEX and BMP-2 on the 1393@MBG scaffold to endow it with antibacterial and osteogenic properties. Results indicated that this 1393@MBG scaffold could effectively load and controlled release BMP-2, DNA and DEX, which can be applied for orthopedic treatment. The in vitro study showed that the DEX loaded 1393@MBG exhibited excellent antibacterial ability, which was evaluated by Staphylococcus aureus (S. aureus), and the BMP-2 loaded 1393@MBG can improve the alkaline phosphatase (ALP) activity and upregulate the expression of osteogenesis-related genes (OCN and RUNX2) of human bone mesenchymal stem cells (hBMSCs). Moreover, the in vivo study further confirmed that the BMP-2 loaded 1393@MBG group showed better osteogenic capacity as compared to that of the 1393 group in a rat femoral defect. Together, these results suggested that the vehicle-like drug delivery 3D printing scaffold 1393@MBG could be a promising candidate for bone repair and relative bone disease treatment.

Keywords: vehicle-like 3D printing scaffold, control release, mesoporous bioactive glass, 1393 bioactive glass, bone treatment