Int J Biol Sci 2021; 17(8):1864-1877. doi:10.7150/ijbs.59725

Research Paper

Could protein content of Urinary Extracellular Vesicles be useful to detect Cirrhosis in Alcoholic Liver Disease?

Esperanza Gonzalez1✉, Mikel Azkargorta2, Clara Garcia-Vallicrosa1, Janire Prieto-Elordui3, Felix Elortza2, Sonia Blanco-Sampascual3, Juan Manuel Falcon-Perez1,4,5✉

1. Exosomes Laboratory. Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Derio, Spain.
2. Proteomics Platform. Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Derio, Spain.
3. Servicio de Aparato Digestivo, Hospital Universitario de Basurto, Bilbao, Spain.
4. Centro de Investigación Biomédica en Red de enfermedades hepáticas y digestivas (CIBERehd), Madrid, Spain.
5. IKERBASQUE Basque Foundation for Science Bilbao Spain.

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Citation:
Gonzalez E, Azkargorta M, Garcia-Vallicrosa C, Prieto-Elordui J, Elortza F, Blanco-Sampascual S, Falcon-Perez JM. Could protein content of Urinary Extracellular Vesicles be useful to detect Cirrhosis in Alcoholic Liver Disease?. Int J Biol Sci 2021; 17(8):1864-1877. doi:10.7150/ijbs.59725. Available from https://www.ijbs.com/v17p1864.htm

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Abstract

Alcohol abuse has a high impact on the mortality and morbidity related to a great number of diseases and is responsible for the development of alcoholic liver disease (ALD). It remains challenging to detect and evaluate its severity, which is crucial for prognosis. In this work, we studied if urinary EVs (uEVs) could serve in diagnose and evaluate cirrhosis in ALD. To this purpose, uEVs characterization by cryo-electron microscopy (Cryo-EM), Nanoparticle Tracking Analysis (NTA) and Western blotting (WB) was performed in a cohort of 21 controls and 21 cirrhotic patients. Then, proteomics of uEVs was carried out in a second cohort of 6 controls and 8 patients in order to identify new putative biomarkers for cirrhosis in ALD. Interestingly, uEVs concentration, size and protein composition were altered in cirrhotic patients. From a total of 1304 proteins identified in uEVs, 90 of them were found to be altered in cirrhotic patients. The results suggest that uEVs could be considered as a tool and a supplier of new biomarkers for cirrhosis in ALD, whose application would be especially relevant in chronic patients. Yet, further research is necessary to obtain more relevant result in clinical terms.

Keywords: urinary extracellular vesicles (uEVs), biomarkers, liquid biopsy, cirrhosis, fibrosis, alcoholic liver disease (ALD)