Int J Biol Sci 2023; 19(3):881-896. doi:10.7150/ijbs.77677 This issue Cite

Research Paper

Ubiquitin like protein FAT10 repressed cardiac fibrosis after myocardial ischemic via mediating degradation of Smad3 dependent on FAT10-proteasome system

Chen Chen1,2*, Xiaoqing Li1,2*, Tao Zhou2*, Yuhao Su1,2, Bodong Yu4, Jiejing Jin2, Jinyan Xie2, Yang Shen2,3, Rong Wan2✉, Kui Hong1,2,3✉

1. Department of Cardiovascular Medicine, the Second Affiliated Hospital of Nanchang University, Nanchang of Jiangxi, 330006 China
2. Jiangxi Key Laboratory of Molecular Medicine, Nanchang of Jiangxi, 330006 China
3. Department of Genetic Medicine, the Second Affiliated Hospital of Nanchang University, Nanchang of Jiangxi, 330006 China
4. Second College of Clinical Medicine, Nanchang University, Nanchang, Jiangxi, 330006 China
*These authors contributed equally to the manuscript

Citation:
Chen C, Li X, Zhou T, Su Y, Yu B, Jin J, Xie J, Shen Y, Wan R, Hong K. Ubiquitin like protein FAT10 repressed cardiac fibrosis after myocardial ischemic via mediating degradation of Smad3 dependent on FAT10-proteasome system. Int J Biol Sci 2023; 19(3):881-896. doi:10.7150/ijbs.77677. https://www.ijbs.com/v19p0881.htm
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Abstract

Graphic abstract

Cardiac fibrosis after myocardial ischemic (MI) injury is a key factor in heart function deterioration. We recently showed that ubiquitin-like protein human HLA-F adjacent transcript (FAT10) plays a novel role in ischemic cardiovascular diseases, but its function in cardiac fibrosis remains unknown. The present study aims to detail the pathophysiological function of FAT10 in MI injury-induced cardiac fibrosis and its underlying mechanism. In vivo, a systemic FAT10 deficiency mouse (Fat10-/-) model was established which exhibited excessive cardiac fibrosis and deleterious cardiac function after MI when compared to wild-type mice. Cardiac fibrotic-related proteins (α-SMA, collagen I and collagen III) content were increased in MI-Fat10-/- mice. Similarly, cardiac FAT10 restoration in Fat10-/- mice suppressed fibrosis and improved cardiac function. In vitro, FAT10 overexpression exert a protective effect against the transforming growth β1 (TGF-β1)-induced proliferation, migration and differentiation in cardiac fibroblast (CFs), primary CFs from Fat10-/- mice and human induced pluripotent stem cell-derived CFs (hiPSC-CFs). Furthermore, immunoprecipitation-mass spectrometry (IP-MS) data demonstrated that FAT10 might mediate Smad3, a critical factor in cardiac fibrosis. Combined with rescue assays both in vivo and vitro, the protective effects of FAT10 against cardiac fibrosis was detected to be dependent on Smad3. In depth, Smad3 as a FAT10 specific substrate, FAT10 specifically bind to the K378 site of Smad3 directly via its C-terminal glycine residues and mediated the degradation of Smad3 through the FAT10-proteasome system instead of ubiquitin. In conclusion, we here show that FAT10 is a novel regulator against cardiac fibrosis after MI by mediating Smad3 degradation through FAT10-mediated proteasome system. Our study confirms the cardioprotective role of FAT10 in the heart, and providing a new prospective insight into the regulation of cardiac fibrosis after MI.

Keywords: FAT10, Cardiac fibrosis, Myocardial infarction, Smad3, HiPSC-CFs


Citation styles

APA
Chen, C., Li, X., Zhou, T., Su, Y., Yu, B., Jin, J., Xie, J., Shen, Y., Wan, R., Hong, K. (2023). Ubiquitin like protein FAT10 repressed cardiac fibrosis after myocardial ischemic via mediating degradation of Smad3 dependent on FAT10-proteasome system. International Journal of Biological Sciences, 19(3), 881-896. https://doi.org/10.7150/ijbs.77677.

ACS
Chen, C.; Li, X.; Zhou, T.; Su, Y.; Yu, B.; Jin, J.; Xie, J.; Shen, Y.; Wan, R.; Hong, K. Ubiquitin like protein FAT10 repressed cardiac fibrosis after myocardial ischemic via mediating degradation of Smad3 dependent on FAT10-proteasome system. Int. J. Biol. Sci. 2023, 19 (3), 881-896. DOI: 10.7150/ijbs.77677.

NLM
Chen C, Li X, Zhou T, Su Y, Yu B, Jin J, Xie J, Shen Y, Wan R, Hong K. Ubiquitin like protein FAT10 repressed cardiac fibrosis after myocardial ischemic via mediating degradation of Smad3 dependent on FAT10-proteasome system. Int J Biol Sci 2023; 19(3):881-896. doi:10.7150/ijbs.77677. https://www.ijbs.com/v19p0881.htm

CSE
Chen C, Li X, Zhou T, Su Y, Yu B, Jin J, Xie J, Shen Y, Wan R, Hong K. 2023. Ubiquitin like protein FAT10 repressed cardiac fibrosis after myocardial ischemic via mediating degradation of Smad3 dependent on FAT10-proteasome system. Int J Biol Sci. 19(3):881-896.

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