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Int J Biol Sci 2023; 19(4):1080-1093. doi:10.7150/ijbs.72889 This issue Cite

Research Paper

Inactivation of Exosc10 in the oocyte impairs oocyte development and maturation, leading to a depletion of the ovarian reserve in mice

Leïla Demini1, Christine Kervarrec1*, Laëtitia Guillot1,2*, Emmanuelle Com1,2, Régis Lavigne1,2, Pierre-Yves Kernanec1, Michael Primig1, Charles Pineau1,2, Fabrice G. Petit1✉, Soazik P. Jamin1✉

1. Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail) - UMR_S 1085, F-35000 Rennes, France.
2. Univ Rennes, CNRS, Inserm, Biosit UAR 3480 US 018, Protim core facility, F-35000 Rennes, France.
* These authors contributed equally.

✉ Corresponding authors: Contributed equally to this work. E-mail: soazik.jaminfr; fabrice.petitfr.

Citation:
Demini L, Kervarrec C, Guillot L, Com E, Lavigne R, Kernanec PY, Primig M, Pineau C, Petit FG, Jamin SP. Inactivation of Exosc10 in the oocyte impairs oocyte development and maturation, leading to a depletion of the ovarian reserve in mice. Int J Biol Sci 2023; 19(4):1080-1093. doi:10.7150/ijbs.72889. https://www.ijbs.com/v19p1080.htm
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Abstract

Graphic abstract

EXOSC10 is a catalytic subunit of the nuclear RNA exosome, and possesses a 3'-5' exoribonuclease activity. The enzyme processes and degrades different classes of RNAs. To delineate the role of EXOSC10 during oocyte growth, specific Exosc10 inactivation was performed in oocytes from the primordial follicle stage onward using the Gdf9-iCre; Exosc10f/- mouse model (Exosc10cKO(Gdf9)). Exosc10cKO(Gdf9) female mice are infertile. The onset of puberty and the estrus cycle in mutants are initially normal and ovaries contain all follicle classes. By the age of eight weeks, vaginal smears reveal irregular estrus cycles and mutant ovaries are completely depleted of follicles. Mutant oocytes retrieved from the oviduct are degenerated, and occasionally show an enlarged polar body, which may reflect a defective first meiotic division. Under fertilization conditions, the mutant oocytes do not enter into an embryonic development process. Furthermore, we conducted a comparative proteome analysis of wild type and Exosc10 knockout mouse ovaries, and identified EXOSC10-dependent proteins involved in many biological processes, such as meiotic cell cycle progression and oocyte maturation. Our results unambiguously demonstrate an essential role for EXOSC10 in oogenesis and may serve as a model for primary ovarian insufficiency in humans. Data are available via ProteomeXchange with identifier PXD039417.

Keywords: Exosc10, oogenesis, follicular development, ovary

Citation styles

APA
Demini, L., Kervarrec, C., Guillot, L., Com, E., Lavigne, R., Kernanec, P.Y., Primig, M., Pineau, C., Petit, F.G., Jamin, S.P. (2023). Inactivation of Exosc10 in the oocyte impairs oocyte development and maturation, leading to a depletion of the ovarian reserve in mice. International Journal of Biological Sciences, 19(4), 1080-1093. https://doi.org/10.7150/ijbs.72889.

ACS
Demini, L.; Kervarrec, C.; Guillot, L.; Com, E.; Lavigne, R.; Kernanec, P.Y.; Primig, M.; Pineau, C.; Petit, F.G.; Jamin, S.P. Inactivation of Exosc10 in the oocyte impairs oocyte development and maturation, leading to a depletion of the ovarian reserve in mice. Int. J. Biol. Sci. 2023, 19 (4), 1080-1093. DOI: 10.7150/ijbs.72889.

NLM
Demini L, Kervarrec C, Guillot L, Com E, Lavigne R, Kernanec PY, Primig M, Pineau C, Petit FG, Jamin SP. Inactivation of Exosc10 in the oocyte impairs oocyte development and maturation, leading to a depletion of the ovarian reserve in mice. Int J Biol Sci 2023; 19(4):1080-1093. doi:10.7150/ijbs.72889. https://www.ijbs.com/v19p1080.htm

CSE
Demini L, Kervarrec C, Guillot L, Com E, Lavigne R, Kernanec PY, Primig M, Pineau C, Petit FG, Jamin SP. 2023. Inactivation of Exosc10 in the oocyte impairs oocyte development and maturation, leading to a depletion of the ovarian reserve in mice. Int J Biol Sci. 19(4):1080-1093.

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