Int J Biol Sci 2023; 19(6):1664-1680. doi:10.7150/ijbs.78864 This issue Cite

Research Paper

Cardiovascular-related proteomic changes in ECFCs exposed to the serum of COVID-19 patients

Lucía Beltrán-Camacho1,2, Santosh D. Bhosale3, Daniel Sánchez-Morillo1,4, Ismael Sánchez-Gomar1,2, Marta Rojas-Torres1,2, Sara Eslava-Alcón1,2, Mario Martínez-Torija5, Mª Angeles Ruiz de Infante6, Mª Dolores Nieto-Martín7, Manuel A. Rodríguez-Iglesias1,2,8, Juan A. Moreno9,10, Esther Berrocoso2,11,12, Martin R. Larsen3, Rafael Moreno-Luna13,14*✉, Mª Carmen Durán-Ruiz1,2*✉

1. Biomedicine, Biotechnology and Public Health Department, University of Cadiz, 11002 Cadiz, Spain
2. Biomedical Research and Innovation Institute of Cadiz (INiBICA), 11002 Cadiz, Spain
3. Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense, Denmark
4. Automation Engineering, Electronics and Computer Architecture and Networks Department, University of Cadiz, 11009 Cadiz, Spain
5. Department of Nursing, National Paraplegic Hospital, Toledo, Spain
6. Occupational Health Service, National Paraplegic Hospital, SESCAM, 45071, Toledo, Spain
7. Internal Medicine Department, University Hospital Virgen del Rocío, Seville, Spain
8. UGC Microbiología, University Hospital Puerta del Mar, Avda. Ana de Viya 21, 11009, Cádiz, Spain
9. Cell Biology, Physiology and Immunology Department, Agrifood Campus of International Excellence (ceiA3), University of Cordoba, 14014, Córdoba, Spain
10. Maimonides Biomedical Research Institute of Cordoba (IMIBIC), UGC Nephrology, Hospital Universitario Reina Sofia, 14004, Cordoba, Spain
11. Psychology Department, University of Cádiz, 11510, Puerto Real, Spain
12. Biomedical Research Networking Center for Mental Health Network (CIBERSAM), Institute of Health Carlos III, 28029, Madrid, Spain
13. Laboratory of Neuroinflammation, National Paraplegic Hospital, 45071 Toledo, Spain
14. Redes de investigación cooperativa orientadas a resultados en salud, Enfermedades vasculares cerebrales, RICORS-ICTUS, SESCAM
*Both authors have equally contributed to this work.

Citation:
Beltrán-Camacho L, Bhosale SD, Sánchez-Morillo D, Sánchez-Gomar I, Rojas-Torres M, Eslava-Alcón S, Martínez-Torija M, de Infante MAR, Nieto-Martín MD, Rodríguez-Iglesias MA, Moreno JA, Berrocoso E, Larsen MR, Moreno-Luna R, Durán-Ruiz MC. Cardiovascular-related proteomic changes in ECFCs exposed to the serum of COVID-19 patients. Int J Biol Sci 2023; 19(6):1664-1680. doi:10.7150/ijbs.78864. https://www.ijbs.com/v19p1664.htm
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Abstract

Graphic abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection significantly affects the cardiovascular system, causing vascular damage and thromboembolic events in critical patients. Endothelial dysfunction represents one of the first steps in response to COVID-19 that might lead to cardiovascular complications and long-term sequelae. However, despite the enormous efforts in the last two years, the molecular mechanisms involved in such processes remain poorly understood. Herein, we analyzed the protein changes taking place in endothelial colony forming cells (ECFCs) after the incubation with the serum from individuals infected with COVID-19, whether asymptomatic or critical patients, by application of a label free-quantitative proteomics approach. Specifically, ECFCs from healthy individuals were incubated ex-vivo with the serum of either COVID-19 negative donors (PCR-/IgG-, n:8), COVID-19 asymptomatic donors at different infective stages (PCR+/ IgG-, n:8and PCR-/IgG+, n:8), or hospitalized critical COVID-19 patients (n:8), followed by proteomics analysis. In total, 590 proteins were differentially expressed in ECFCs in response to all infected serums. Predictive analysis highlighted several proteins like CAPN5, SURF4, LAMP2 or MT-ND1, as highly discriminating features between the groups compared. Protein changes correlated with viral infection, RNA metabolism or autophagy, among others. Remarkably, the angiogenic potential of ECFCs in response to the infected serums was impaired, and many of the protein alterations in response to the serum of critical patients were associated with cardiovascular-related pathologies.

Keywords: COVID-19, ECFCs, Viral infection, RNA metabolism, cardiovascular diseases, endothelial dysfunction, autophagy, proteomics, mass spectrometry


Citation styles

APA
Beltrán-Camacho, L., Bhosale, S.D., Sánchez-Morillo, D., Sánchez-Gomar, I., Rojas-Torres, M., Eslava-Alcón, S., Martínez-Torija, M., de Infante, M.A.R., Nieto-Martín, M.D., Rodríguez-Iglesias, M.A., Moreno, J.A., Berrocoso, E., Larsen, M.R., Moreno-Luna, R., Durán-Ruiz, M.C. (2023). Cardiovascular-related proteomic changes in ECFCs exposed to the serum of COVID-19 patients. International Journal of Biological Sciences, 19(6), 1664-1680. https://doi.org/10.7150/ijbs.78864.

ACS
Beltrán-Camacho, L.; Bhosale, S.D.; Sánchez-Morillo, D.; Sánchez-Gomar, I.; Rojas-Torres, M.; Eslava-Alcón, S.; Martínez-Torija, M.; de Infante, M.A.R.; Nieto-Martín, M.D.; Rodríguez-Iglesias, M.A.; Moreno, J.A.; Berrocoso, E.; Larsen, M.R.; Moreno-Luna, R.; Durán-Ruiz, M.C. Cardiovascular-related proteomic changes in ECFCs exposed to the serum of COVID-19 patients. Int. J. Biol. Sci. 2023, 19 (6), 1664-1680. DOI: 10.7150/ijbs.78864.

NLM
Beltrán-Camacho L, Bhosale SD, Sánchez-Morillo D, Sánchez-Gomar I, Rojas-Torres M, Eslava-Alcón S, Martínez-Torija M, de Infante MAR, Nieto-Martín MD, Rodríguez-Iglesias MA, Moreno JA, Berrocoso E, Larsen MR, Moreno-Luna R, Durán-Ruiz MC. Cardiovascular-related proteomic changes in ECFCs exposed to the serum of COVID-19 patients. Int J Biol Sci 2023; 19(6):1664-1680. doi:10.7150/ijbs.78864. https://www.ijbs.com/v19p1664.htm

CSE
Beltrán-Camacho L, Bhosale SD, Sánchez-Morillo D, Sánchez-Gomar I, Rojas-Torres M, Eslava-Alcón S, Martínez-Torija M, de Infante MAR, Nieto-Martín MD, Rodríguez-Iglesias MA, Moreno JA, Berrocoso E, Larsen MR, Moreno-Luna R, Durán-Ruiz MC. 2023. Cardiovascular-related proteomic changes in ECFCs exposed to the serum of COVID-19 patients. Int J Biol Sci. 19(6):1664-1680.

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