Int J Biol Sci 2020; 16(12):2051-2062. doi:10.7150/ijbs.44119
RNF8 induces β-catenin-mediated c-Myc expression and promotes colon cancer proliferation
1. Department of Anorectal Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, RP China
2. Institute of Translational Medicine, College of Basic Medicine, China Medical University, Shenyang 110122, RP China
3. Panjin Liaohe Oilfield Gem FLower Hospital, Panjin 7650036, RP China
4. Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical University, Shenyang 110122, RP China
5. Department of Neurosurgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, RP China
*These authors contributed equally to this work.
Ren L, Zhou T, Wang Y, Wu Y, Xu H, Liu J, Dong X, Yi F, Guo Q, Wang Z, Li X, Bai N, Guo W, Guo M, Jiang B, Wu X, Feng Y, Song X, Zhang S, Zhao Y, Cao L, Han S, Xing C. RNF8 induces β-catenin-mediated c-Myc expression and promotes colon cancer proliferation. Int J Biol Sci 2020; 16(12):2051-2062. doi:10.7150/ijbs.44119. Available from http://www.ijbs.com/v16p2051.htm
DNA damage signals transducer RING finger protein 8 (RNF8) is involved in maintaining genomic stability by facilitating the repair of DNA double-strand breaks (DSB) via ubiquitin signaling. By analyzing the TCGA database and colon cancer tissue microarrays, we found that the expression level of RNF8 was positively correlated with that of c-Myc in colon cancer, which were closely associated with poor survival of colon cancer patients. Furthermore, overexpressing and knocking down RNF8 increased and decreased the expression of c-Myc in colon cancer cells, respectively. In addition, RNF8 interacted with β-catenin and facilitated its nuclear translocation by conjugating K63 polyubiquitination on it. These observations suggested a de novo role of RNF8 in promoting the progression of colon cancer by inducing β-catenin-mediated c-Myc expression.
Keywords: RNF8, c-Myc, β-catenin, colon cancer