Full Text | PDF

Share on tweeters Share on facebook Share on linkedin Share on googleplus

Int J Biol Sci 2020; 16(13):2283-2295. doi:10.7150/ijbs.33481 This issue Cite

Review

CRISPR/Cas9-related technologies in liver diseases: from feasibility to future diversity

Tao Xu1,2*, Li Li3,4*, Yu-chen Liu5*, Wei Cao6*, Jia-si Chen1,2, Shuang Hu1,2, Ying Liu7, Liang-yun Li1,2, Hong Zhou1,2,9, Xiao-ming Meng1,2, Cheng Huang1,2, Lei Zhang1,2, Jun Li1,2✉, Huan Zhou8✉

1. Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, Anhui Institute of Innovative Drugs, School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei 230032, Anhui Province, China.
2. Institute for Liver Diseases of Anhui Medical University, Anhui Medical University, Hefei 230032, China.
3. Department of Pathology and Pathophysiology, and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
4. Institute of Hematology, Zhejiang University & Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou, China.
5. Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen 518039, China.
6. Shenzhen Qianhai Icecold IT Co., Ltd. China.
7. Hefei Institutes of Physical Science, Chinese Academy of Sciences; Anhui Province Key Laboratory of Environmental Toxicology and Pollution Control Technology, Hefei, Anhui, PR China.
8. National Drug Clinical Trial Institution, the First Affiliated Hospital of Bengbu Medical College.
9. Anhui Provincial Cancer Hospital, West Branch of The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230031, P.R. China.
*These authors contributed equally to this work.

✉ Corresponding authors: Jun Li, PhD. School of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei, Anhui Province, 230032, China. Tel./Fax: +86 551 65161001. E-mail: ljedu.cn; Huan Zhou, PhD, MD. National Drug Clinical Trial Institution More

Citation:
Xu T, Li L, Liu Yc, Cao W, Chen Js, Hu S, Liu Y, Li Ly, Zhou H, Meng Xm, Huang C, Zhang L, Li J, Zhou H. CRISPR/Cas9-related technologies in liver diseases: from feasibility to future diversity. Int J Biol Sci 2020; 16(13):2283-2295. doi:10.7150/ijbs.33481. https://www.ijbs.com/v16p2283.htm
Other styles

File import instruction

Abstract

Graphic abstract

Liver diseases are one of the leading causes of mortality in the world, mainly caused by different etiological agents, alcohol consumption, viruses, drug intoxication, and malnutrition. The maturation of gene therapy has heralded new avenues for developing effective interventions for these diseases. Derived from a remarkable microbial defense system, clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins 9 system (CRISPR/Cas9 system) is driving innovative applications from basic biology to biotechnology and medicine. Recently, the mutagenic function of CRISPR/Cas9 system has been widely adopted for genome and disease research. In this review, we describe the development and applications of CRISPR/Cas9 system on liver diseases for research or translational applications, while highlighting challenges as well as future avenues for innovation.

Keywords: CRISPR/Cas9, viral hepatitis, hepatocellular carcinoma (HCC), clinical research

Citation styles

APA
Xu, T., Li, L., Liu, Y.c., Cao, W., Chen, J.s., Hu, S., Liu, Y., Li, L.y., Zhou, H., Meng, X.m., Huang, C., Zhang, L., Li, J., Zhou, H. (2020). CRISPR/Cas9-related technologies in liver diseases: from feasibility to future diversity. International Journal of Biological Sciences, 16(13), 2283-2295. https://doi.org/10.7150/ijbs.33481.

ACS
Xu, T.; Li, L.; Liu, Y.c.; Cao, W.; Chen, J.s.; Hu, S.; Liu, Y.; Li, L.y.; Zhou, H.; Meng, X.m.; Huang, C.; Zhang, L.; Li, J.; Zhou, H. CRISPR/Cas9-related technologies in liver diseases: from feasibility to future diversity. Int. J. Biol. Sci. 2020, 16 (13), 2283-2295. DOI: 10.7150/ijbs.33481.

NLM
Xu T, Li L, Liu Yc, Cao W, Chen Js, Hu S, Liu Y, Li Ly, Zhou H, Meng Xm, Huang C, Zhang L, Li J, Zhou H. CRISPR/Cas9-related technologies in liver diseases: from feasibility to future diversity. Int J Biol Sci 2020; 16(13):2283-2295. doi:10.7150/ijbs.33481. https://www.ijbs.com/v16p2283.htm

CSE
Xu T, Li L, Liu Yc, Cao W, Chen Js, Hu S, Liu Y, Li Ly, Zhou H, Meng Xm, Huang C, Zhang L, Li J, Zhou H. 2020. CRISPR/Cas9-related technologies in liver diseases: from feasibility to future diversity. Int J Biol Sci. 16(13):2283-2295.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Popup Image

©2024 Ivyspring International Publisher. Terms of use