Int J Biol Sci 2008; 4(6):362-367. doi:10.7150/ijbs.4.362
Association of Insertion/Deletion Polymorphism of Alpha-Adrenoceptor Gene in Essential Hypertension with or without Type 2 Diabetes Mellitus in Malaysian Subjects
1. Molecular Biology Lab, Department of Biomedical Science, Universiti Putra Malaysia, Serdang 43400, Malaysia
2. Department of Medicine, Faculty of Medicine and Health Sciences, Serdang 43400, Malaysia
Vasudevan R, Ismail P, Stanslas J, Shamsudin N, Ali Ab. Association of Insertion/Deletion Polymorphism of Alpha-Adrenoceptor Gene in Essential Hypertension with or without Type 2 Diabetes Mellitus in Malaysian Subjects. Int J Biol Sci 2008; 4(6):362-367. doi:10.7150/ijbs.4.362. Available from http://www.ijbs.com/v04p0362.htm
An insertion/deletion (I/D) polymorphism of Alpha2B-Adrenoceptor (ADRA2B) gene located on chromosome 2 has been studied extensively in related to cardiovascular diseases. The main aim of the present study was to examine the potential association of D allele frequency of I/D polymorphism of ADRA2B gene in Malaysian essential hypertensive subjects with or without type 2 diabetes mellitus (T2DM). This study includes 70 hypertensive subjects without T2DM, 65 hypertensive subjects with T2DM and 75 healthy volunteers as control subjects. Genotyping of I/D polymorphism was performed by conventional PCR method. There was significant difference found in age, body mass index, systolic/diastolic blood pressure and high density lipoprotein cholesterol level between the case and control subjects. DD genotypic frequency of I/D polymorphism was significantly higher in hypertensive subjects (42.84% vs. 29.33%; P=0.029) and in hypertensive with T2DM subjects (46.15% vs. 29.33%; P=0.046) than control group. D allele frequency was higher in hypertensive group (67.41%) than control subjects (52.67%). However, no significant difference was found between the three genotypes of I/D polymorphism of ADRA2B gene and the clinical characteristics of the subjects. The result obtained in this study show D allele of ADRA2B gene was associated with essential hypertension with or without T2DM in Malaysian subjects.
Keywords: Alpha2B-Adrenoceptor, Essential hypertension, Type 2 Diabetes Mellitus, Insertion/Deletion polymorphism