Full Text | PDF

Int J Biol Sci 2009; 5(4):311-318. doi:10.7150/ijbs.5.311 This issue Cite

Research Paper

Body fat mass reduction and up-regulation of uncoupling protein by novel lipolysis-promoting plant extract

Shinobu Mori^{1,2 ✉}, Mayumi Satou¹, Satoshi Kanazawa¹, Naonobu Yoshizuka¹, Tadashi Hase¹, Ichiro Tokimitsu¹, Yoshinori Takema¹, Yoshinori Nishizawa¹, Toshihiko Yada²

1. Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikai-Machi, Haga-gun, Tochigi, 321-3497, Japan
2. Department of Physiology, Division of Integrative Physiology, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan

✉ Correspondence to: Shinobu Mori, Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikai-Machi, Haga-gun, Tochigi, 321-3497, Japan. Tel: +81-285-68-7613; Fax: +81-285-68-7495; E-mail: mori.shinobuco.jp

Abstract

We have found natural products exhibiting lipolysis-promoting activity in subcutaneous adipocytes, which are less sensitive to hormones than visceral adipocytes. The activities and a action mechanisms of a novel plant extract of Cirsium oligophyllum (CE) were investigated in isolated adipocytes from rat subcutaneous fat, and its fat-reducing effects by peroral administration and topical application were evaluated in vivo. CE-induced lipolysis was synergistically enhanced by caffeine, a phosphodiesterase inhibitor, and was reduced by propranolol, a β adrenergic antagonist. The peroral administration of 10% CE solution to Wistar rats for 32 days reduced body weight gain, subcutaneous, and visceral fat weights by 6.6, 26.2, and 3.0%, respectively, as compared to the control group. By the topical application of 2% of this extract to rats for 7 days, weight of subcutaneous fat in the treated skin was reduced by 23.2%. This fat mass reduction was accompanied by the up-regulation of uncoupling protein 1 (UCP), a principal thermogenic mitochondrial molecule related to energy dissipating, in subcutaneous fat and UCP3 in skin except for the fat layer. These results indicate that CE promotes lipolysis via a mechanism involving the β adrenergic receptor, and affects the body fat mass. This fat reduction may be partially due to UCP up-regulation in the skin including subcutaneous fat. This is the first report showing that repeated lipolysis promotion through CE administration may be beneficial for the systematic suppression of body fat accumulation or the control of fat distribution in obesity.

Keywords: β adrenergic receptor, lipolysis, fat reduction, uncoupling protein, plant extract

Citation styles

©2024 Ivyspring International Publisher. Terms of use