Int J Biol Sci
2011; 7(2):168-179.
doi:10.7150/ijbs.7.168 This issueCite
Research Paper
Gamma-ray Irradiation Impairs Dendritic Cell Migration to CCL19 by Down-regulation of CCR7 and Induction of Cell Apoptosis
Cong Liu*, Jin Lin*, Luqian Zhao*, Yanyong Yang, Fu Gao, Bailong Li, Jianguo Cui✉, Jianming Cai✉
Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, PR China. * These authors contributed equally to this work.
✉ Corresponding author: Jianming Cai and Jianguo Cui. Address: Department of Radiation Medicine, Second Military Medical University; 800, Xiangyin Road 200433, Shanghai; P.R. China Fax: +86-21-81871148. E-mail: caijianming882003com and mustardcccom.cn.
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Citation:
Liu C, Lin J, Zhao L, Yang Y, Gao F, Li B, Cui J, Cai J. Gamma-ray Irradiation Impairs Dendritic Cell Migration to CCL19 by Down-regulation of CCR7 and Induction of Cell Apoptosis. Int J Biol Sci 2011; 7(2):168-179. doi:10.7150/ijbs.7.168. https://www.ijbs.com/v07p0168.htm
Dendritic cells (DCs) are the most potent antigen-presenting cells and play a crucial role in the regulation of immune response and migration of DCs into secondary lymphoid tissues also play an important role in the initiation of innate and adaptive immunity. Radiation therapy is now a routine treatment for certain types of cancer and over 20 percent of cancer patients will require radiation therapy during the treatment of their disease. However, the influence of ionizing irradiation on the migratory ability of DCs is largely unknown. In this article, we report that γ ray irradiation can significantly inhibit LPS-triggered up regulation of CCR7 expression and PGE2 production by DC, thus impairing DC migration towards CCL19 in vitro and in vivo. Moreover, γ ray exposed DC also displayed an increased apoptosis rate and decreased cell viability. Furthermore, we demonstrate that exogenous PGE2 can partly reduce the gamma-ray induced migratory impairment and restored CCR7 expression of DC. Our work suggests that γ irradiation affects DC function at multiple steps during the immune response including DC migration, and that PGE2, via control of CCR7 expression, is an important regulator of DC migration.
Keywords: γ ray Irradiation, Dendritic cell, Migration, CCR7, Apoptosis.
Citation styles
APA
Liu, C., Lin, J., Zhao, L., Yang, Y., Gao, F., Li, B., Cui, J., Cai, J. (2011). Gamma-ray Irradiation Impairs Dendritic Cell Migration to CCL19 by Down-regulation of CCR7 and Induction of Cell Apoptosis. International Journal of Biological Sciences, 7(2), 168-179. https://doi.org/10.7150/ijbs.7.168.
ACS
Liu, C.; Lin, J.; Zhao, L.; Yang, Y.; Gao, F.; Li, B.; Cui, J.; Cai, J. Gamma-ray Irradiation Impairs Dendritic Cell Migration to CCL19 by Down-regulation of CCR7 and Induction of Cell Apoptosis. Int. J. Biol. Sci. 2011, 7 (2), 168-179. DOI: 10.7150/ijbs.7.168.
NLM
Liu C, Lin J, Zhao L, Yang Y, Gao F, Li B, Cui J, Cai J. Gamma-ray Irradiation Impairs Dendritic Cell Migration to CCL19 by Down-regulation of CCR7 and Induction of Cell Apoptosis. Int J Biol Sci 2011; 7(2):168-179. doi:10.7150/ijbs.7.168. https://www.ijbs.com/v07p0168.htm
CSE
Liu C, Lin J, Zhao L, Yang Y, Gao F, Li B, Cui J, Cai J. 2011. Gamma-ray Irradiation Impairs Dendritic Cell Migration to CCL19 by Down-regulation of CCR7 and Induction of Cell Apoptosis. Int J Biol Sci. 7(2):168-179.