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Int J Biol Sci 2011; 7(6):805-814. doi:10.7150/ijbs.7.805 This issue Cite

Research Paper

MicroRNA-7 Inhibits the Growth of Human Non-Small Cell Lung Cancer A549 Cells through Targeting BCL-2

Shudao Xiong*, Yijie Zheng*, Pei Jiang, Ronghua Liu, Xiaoming Liu, Yiwei Chu✉

Department of Immunology, Shanghai Medical College, Key Laboratory of Molecular Medicine of Ministry of Education, Fudan University, Shanghai, China.
* Shudao Xiong and Yijie Zheng contributed equally to this work and should be considered as co-first authors.

✉ Corresponding author: Yiwei Chu, MD, PhD, Department of Immunology, Shanghai Medical College, Fudan University, 138 Yixueyuan Road, mail box 226, Shanghai, 200032, China. Tel/Fax: +86-21-54237324. E-mail: yiwei_chucom

Citation:
Xiong S, Zheng Y, Jiang P, Liu R, Liu X, Chu Y. MicroRNA-7 Inhibits the Growth of Human Non-Small Cell Lung Cancer A549 Cells through Targeting BCL-2. Int J Biol Sci 2011; 7(6):805-814. doi:10.7150/ijbs.7.805. https://www.ijbs.com/v07p0805.htm
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Abstract

MicroRNAs(miRNAs) are emerging as important regulators in tumorigenesis. Increasing evidences have indicated microRNA-7(miR-7) to be a potential tumor suppressor in several human cancers. However, only a limited number of target genes have been identified so far and its biological function in Non-Small Cell Lung Cancer (NSCLC) remains to be further elucidated. In the present study, we observed a reduction of miR-7 level in NSCLC cell lines. Overexpression of miR-7 not only suppressed NSCLC A549 cells proliferation, induced cell apoptosis and inhibited cell migration in vitro, but also reduced tumorigenicity in vivo. Bioinformatics predictions revealed a potential binding site of miR-7 on 3'UTR of BCL-2 and it was further confirmed by luciferase assay. Moreover, subsequent experiments showed that BCL-2 was downregulated by miR-7 at both transcriptional and translational levels. These results suggest that miR-7 regulates the expression of BCL-2 through direct 3'UTR interactions. Therefore, we postulate BCL-2 to be a novel target possibly involved in miR-7-mediated growth suppression and apoptosis of A549 cells. These findings may provide a basic rationale for the use of miR-7 in the treatment of NSCLC.

Keywords: miR-7, BCL-2, non-small cell lung cancer, A549 cells, apoptosis.

Citation styles

APA
Xiong, S., Zheng, Y., Jiang, P., Liu, R., Liu, X., Chu, Y. (2011). MicroRNA-7 Inhibits the Growth of Human Non-Small Cell Lung Cancer A549 Cells through Targeting BCL-2. International Journal of Biological Sciences, 7(6), 805-814. https://doi.org/10.7150/ijbs.7.805.

ACS
Xiong, S.; Zheng, Y.; Jiang, P.; Liu, R.; Liu, X.; Chu, Y. MicroRNA-7 Inhibits the Growth of Human Non-Small Cell Lung Cancer A549 Cells through Targeting BCL-2. Int. J. Biol. Sci. 2011, 7 (6), 805-814. DOI: 10.7150/ijbs.7.805.

NLM
Xiong S, Zheng Y, Jiang P, Liu R, Liu X, Chu Y. MicroRNA-7 Inhibits the Growth of Human Non-Small Cell Lung Cancer A549 Cells through Targeting BCL-2. Int J Biol Sci 2011; 7(6):805-814. doi:10.7150/ijbs.7.805. https://www.ijbs.com/v07p0805.htm

CSE
Xiong S, Zheng Y, Jiang P, Liu R, Liu X, Chu Y. 2011. MicroRNA-7 Inhibits the Growth of Human Non-Small Cell Lung Cancer A549 Cells through Targeting BCL-2. Int J Biol Sci. 7(6):805-814.

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