Int J Biol Sci
2011; 7(7):1037-1044.
doi:10.7150/ijbs.7.1037 This issueCite
Research Paper
Development and Initial Characterization of a HAPPY Panel for Mapping the X. Tropicalis Genome
Zhihua Jiang1,✉, Jennifer J. Michal1, Kenneth B. Beckman2, Jessica B. Lyons3, Ming Zhang1, Zengxiang Pan1, Daniel S. Rokhsar3, Richard M. Harland3,✉
1. Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USA; 2. Biomedical Genomics Center, University of Minnesota, Minneapolis, MN 55455, USA; 3. Department of Molecular & Cell Biology, University of California Berkeley, Berkeley, CA 94720-3200, USA.
✉ Corresponding author: Dr. Zhihua Jiang (jiangzedu) or Dr. Richard M. Harland (harlandedu).
Citation:
Jiang Z, Michal JJ, Beckman KB, Lyons JB, Zhang M, Pan Z, Rokhsar DS, Harland RM. Development and Initial Characterization of a HAPPY Panel for Mapping the X. Tropicalis Genome. Int J Biol Sci 2011; 7(7):1037-1044. doi:10.7150/ijbs.7.1037. https://www.ijbs.com/v07p1037.htm
HAPPY mapping was designed to pursue the analysis of approximately random HAPloid DNA breakage samples using the PolYmerase chain reaction for mapping genomes. In the present study, we improved the method and integrated two other molecular techniques into the process: whole genome amplification and the Sequenom SNP (single nucleotide polymorphism) genotyping assay in order to facilitate whole genome mapping of X. tropicalis. The former technique amplified enough DNA materials to genotype a large number of markers, while the latter allowed for relatively high throughput marker genotyping with multiplex assays on the HAPPY lines. A total of 58 X. tropicalis genes were genotyped on an initial panel of 383 HAPPY lines, which contributed to formation of a working panel of 146 lines. Further genotyping of 29 markers on the working panel led to construction of a HAPPY map for the X. tropicalis genome. We believe that our improved HAPPY method described in the present study has paved the way for the community to map different genomes with a simple, but powerful approach.
Jiang, Z., Michal, J.J., Beckman, K.B., Lyons, J.B., Zhang, M., Pan, Z., Rokhsar, D.S., Harland, R.M. (2011). Development and Initial Characterization of a HAPPY Panel for Mapping the X. Tropicalis Genome. International Journal of Biological Sciences, 7(7), 1037-1044. https://doi.org/10.7150/ijbs.7.1037.
ACS
Jiang, Z.; Michal, J.J.; Beckman, K.B.; Lyons, J.B.; Zhang, M.; Pan, Z.; Rokhsar, D.S.; Harland, R.M. Development and Initial Characterization of a HAPPY Panel for Mapping the X. Tropicalis Genome. Int. J. Biol. Sci. 2011, 7 (7), 1037-1044. DOI: 10.7150/ijbs.7.1037.
NLM
Jiang Z, Michal JJ, Beckman KB, Lyons JB, Zhang M, Pan Z, Rokhsar DS, Harland RM. Development and Initial Characterization of a HAPPY Panel for Mapping the X. Tropicalis Genome. Int J Biol Sci 2011; 7(7):1037-1044. doi:10.7150/ijbs.7.1037. https://www.ijbs.com/v07p1037.htm
CSE
Jiang Z, Michal JJ, Beckman KB, Lyons JB, Zhang M, Pan Z, Rokhsar DS, Harland RM. 2011. Development and Initial Characterization of a HAPPY Panel for Mapping the X. Tropicalis Genome. Int J Biol Sci. 7(7):1037-1044.