1. Beijing Chao-Yang Hospital, Capital Medical University, China
2. Beijing Institute of Respiratory Medicine, China
3. Institute of Biomechanics and Medical Engineering, School of Aerospace, Tsinghua University, China
Corrected-article in Int J Biol Sci, Volume 7, 588
In our paper , the second panel of Figure 1c was mis-pasted. Fig. 1c should be corrected as follows.
(A) Tonic stretch-induced ATII cell death over time (1 min, 5 min, 2 h, and 4h). Cytoplasm of viable cells was stained with calcein-AM (green), and nuclei of nonviable cells were stained with ethidium homodimer-1 (red). Bar = 200 μm. (B) Statistical analysis of stretch-induced cell death over time, the cell death rate did not increase after 5 min. Values were means ± SD (n=8). *P < 0.001 vs. unstretched cells (US); **P < 0.001 vs. S-1m. (C) Cells were stained with annexin V and PI, and analyzed by flow cytometry. AnnexinV-positive/PI-negative (lower right quadrant) represented the early apoptosis, annexinV-positive/PI-positive (upper right quadrant) represented the late apoptosis/necrosis. Apoptosis rate was not sig-nificantly different between US and stretch group (S-5m and S-2h). Values were means ± SD (n=8). (D) Tonic stretch-induced cell morphological remodeling was detected by SEM. During the stretch, cells experienced expansion (1 min), contraction (5 min), and reexpansion (2 h). Bar = 20 μm. (E) Stretch induced cell morphological remodeling. Im-munofluorescence of representative cells at the time indicated for actin (red), unclear (blue), and FA marker VIN (green). Bar = 10 μm. (F) Quantitative analysis of FAs fluorescence intensity during the stretch showed that cells experienced rapid FAs disassembly and sequential reassembly. * P < 0.01 vs. US; ** P < 0.01 vs. S-5m. Values were means ± SD (n>60).
All the authors have read the erratum and agree with the correction.
1. Liu XY, Chen XF, Ren YH, Zhan QY, Wang C, Yang C. Alveolar Type II Cells Escape Stress Failure Caused by Tonic Stretch through Transient Focal Adhesion Disassembly. Int J Biol Sci. 2011;7(5):588-599
Corresponding authors: Dr. Chen Wang, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020 (China). Dr. Chun Yang, IBME, School of Aerospace, Tsinghua University, Beijing 100084 (China). Tel. +86 10 8513 2114; Fax +86 10 6506 0167; E-Mail: cyhbirmccmu.edu.cn or yangchunedu.cn