Int J Biol Sci 2022; 18(13):4809-4823. doi:10.7150/ijbs.75084 This issue Cite

Research Paper

The Novel Role of hnRNP UL1 in Human Cell Nucleoli

Marlena Cichocka1,2, Anna Karlik1,2, Patrycja Plewka1,2, Kishor Gawade1,2, Agata Stępień1, Patrycja Świergiel1,2, Ankur Gadgil1,2, Katarzyna Dorota Raczyńska1,2✉

1. Laboratory of RNA Processing, Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Uniwersytetu Poznańskiego 6, 61-614 Poznan, Poland
2. Center for Advanced Technology, Adam Mickiewicz University, Uniwersytetu Poznańskiego 10, 61-614 Poznan, Poland

Citation:
Cichocka M, Karlik A, Plewka P, Gawade K, Stępień A, Świergiel P, Gadgil A, Raczyńska KD. The Novel Role of hnRNP UL1 in Human Cell Nucleoli. Int J Biol Sci 2022; 18(13):4809-4823. doi:10.7150/ijbs.75084. https://www.ijbs.com/v18p4809.htm
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Abstract

Graphic abstract

hnRNP UL1 plays an important role in cell nuclei, where it is recruited to DNA damage sites and is involved in the repair of DNA double-strand breaks. Furthermore, this protein is known as a transcriptional repressor of RNA polymerase II genes. In the present study, we have shown that hnRNP UL1 is also localized in the nucleoli of human cells. Upon investigating its function, we found that hnRNP UL1 stimulates ribosomal DNA (rDNA) gene transcription. Moreover, we observed that cells with hnRNP UL1 silencing exhibited increased sensitivity to DNA damage. We also showed that hnRNP UL1 interacts with γH2A.X, RPA32, XRCC1, and Chk1 in cell nucleoli, suggesting its involvement in the repair of rDNA damage.

Keywords: hnRNP UL1, nucleolus, rDNA genes, transcription, rDNA damage, rDNA repair


Citation styles

APA
Cichocka, M., Karlik, A., Plewka, P., Gawade, K., Stępień, A., Świergiel, P., Gadgil, A., Raczyńska, K.D. (2022). The Novel Role of hnRNP UL1 in Human Cell Nucleoli. International Journal of Biological Sciences, 18(13), 4809-4823. https://doi.org/10.7150/ijbs.75084.

ACS
Cichocka, M.; Karlik, A.; Plewka, P.; Gawade, K.; Stępień, A.; Świergiel, P.; Gadgil, A.; Raczyńska, K.D. The Novel Role of hnRNP UL1 in Human Cell Nucleoli. Int. J. Biol. Sci. 2022, 18 (13), 4809-4823. DOI: 10.7150/ijbs.75084.

NLM
Cichocka M, Karlik A, Plewka P, Gawade K, Stępień A, Świergiel P, Gadgil A, Raczyńska KD. The Novel Role of hnRNP UL1 in Human Cell Nucleoli. Int J Biol Sci 2022; 18(13):4809-4823. doi:10.7150/ijbs.75084. https://www.ijbs.com/v18p4809.htm

CSE
Cichocka M, Karlik A, Plewka P, Gawade K, Stępień A, Świergiel P, Gadgil A, Raczyńska KD. 2022. The Novel Role of hnRNP UL1 in Human Cell Nucleoli. Int J Biol Sci. 18(13):4809-4823.

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